In addition to molecular scale computations for estimating ligand receptor docking, we adapted the Goldstein Perelson model for FcεRI cross-linking to provide further insight into aggregation states of complexes formed after DF3 binding.
Our combined analyses of the oligomeric species provided valuable insights into aggregation pathways to the formation of two distinct cytotoxic oligomers and into the structural features of the oligomeric species.
Over the past few years, our discovery has allowed extensive characterizations by us and other groups on unrefoldable and insoluble proteins, which not has elucidated previously unknown regimes associated with proteins; but has also provided critical insights into aggregation-causing diseases.
Dynamic light scattering, steady-state fluorescence, NMR diffusometry and cryo-TEM have been used to gain more insight into the aggregation behaviour of LPS from Escherichia coli O55 B5.
In order to gain an insight into the aggregation modes of the NRs in the as-collected precipitates, additional experiments have been done to acquire images of the intact precipitates by placing small pieces of silicon wafer on the bottom of the gold NR mixture solution before introducing NaCl solution.
Various techniques such as light scattering [33, 43], fluorescence spectroscopy [44, 45], NMR [46], specific volume [47, 48], and small-angle neutron scattering (SANS) [49, 50] have been frequently used to gain new insight into the aggregation behavior of these systems.
To gain insight on pigment aggregation phenotype, we compared the ultrastructural characteristics of melanosomes between wild-type and feelgood fish (Fig. 4E G).
Further insight into protein aggregation will not only deepen our understanding of ALS pathogenesis but also may provide novel avenues for therapeutic intervention.
Most importantly, to gain more insight into amylin aggregation dynamics and also to screen potential inhibitors of amylin-mediated cytotoxicity, a cost-effective strategy to acquire sufficiently pure amylin is deemed critical.
For an early and accurate diagnosis of the disease as well as for a better treatment hypothesis, it is essential to get a deeper insight on the aggregation of amyloid-β.
The organization of the compounds in solution below and above the transition temperature was studied, and precious insights in the aggregation mechanisms were obtained.
