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After extrusion the liposomes were harvested and excess pyranine removed through gel filtration using a superdex-25 column pre-equilibrated in inside transport buffer without pyranine.
Proteoliposomes were harvested and resuspended in inside transport buffer (5 mM Hepes pH 6.8, 2 mM MgSO4, 1 mM Pyranine (trisodium 8-hydroxypyrene-1,3,6-trisulfonate 8-hydroxypyrene-1,3,6-trisulfonate 8-hydroxypyrene-1,3,6-trisulfonate 8-hydroxypyrene-1,3,6-trisulfonatehree rounds of freeze thalson liquid nitrogen and then extruded through a 0.4-µm membrane.
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The décor of the new restaurant inside transports diners to another era.
Subjects were positioned inside a transport cage that was adjacent to a second transport cage containing a "stooge" 'animal.
In each of these two transports, two mesh buckets containing either vaccinated or unvaccinated fish (12 fish/bucket) were placed inside the transport tank along with the other tagged and untagged fish, and transported to the release site.
Because of high pHi buffering capacity inside myocytes, transport of HCO3− would have a relatively greater effect on the state of the CO2/HCO3− equilibrium than H+ transport.
Its perimeter is 15 miles long and more than 100 miles of railway exist inside to transport materials.
Notably, the Fano factor is always sub-Poissonian whenever ε eff lies inside the transport window.
Extending past the side panels, this design helps to protect Survivor and the hardware inside during transport.
When the level lies inside the transport window, the cross-correlations are suppressed as p increases independently of γsf.
Network mobility (NEMO) allows various types of in-vehicle networks (e.g., WLANs inside public transport vehicle) to be seamlessly connected to the Internet.
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