This study suggests that the "inside and outside" configuration of cell-sorting can be successfully explained based on the concept of directional cell movement and the SAH.
After excision of this patch into the inside-out configuration and changing the bath solution to the high-K+ pipette solution, the estimated single-channel conductance was 210 pS, as previously reported (8).
Thus, we reproduced their model (Additional files 1, 2), examined the final configurations of cell-sorting, and confirmed that the "inside and outside" configuration was not attained under the "specific adhesive relationship" with the DAH model (Additional File 3).
However, the SAH could not reasonably explain the "inside and outside" configuration of cell-sorting [ 9].
Thus, differences in mobility are important for the "inside and outside" configuration of cell-sorting, while differences in the frequency of the onset of cell movement may not be critically involved, as mentioned in the third paragraph of this section.
To objectively determine whether the aggregates had attained an "inside and outside" configuration using each parameter set, we defined two parameters: CELL DISTRIBUTION RATIO and PERIMETER RATIO.
Glazer and Graner (1992) successfully reproduced the "inside and outside" configuration of cell-sorting by constructing a model in which cell movement was quasi-continuous [ 23, 24].
When several parameter sets with different values of ACTIVATION THRESHOLD for each cell type were examined, the "inside and outside" configuration of cell-sorting could not be reproduced.
These results appeared to be inconsistent with previous findings that differences in the ACTIVATION THRESHOLD caused the "inside and outside" configuration of cell-sorting [ 41].
However, when both homophilic adhesions are stronger than the heterophilic adhesion, which corresponds to the "specific adhesive relationship", the DAH model predicts that the "inside and outside" configuration of cell-sorting does not occur.
Although the Hm preference did not seem to contribute to the "inside and outside" configuration under the above-mentioned conditions, "inside and outside" cell sorting occurred when one cell type had an Hm preference and the other cell type had an Htr preference (Fig. 8c e).
