Inside, the room configurations were not drastically changed.
The EAG1 currents were recorded in the inside-out configuration of the patch-clamp technique in order to expose the intracellular putative ligand binding domains to the library compounds.
After excision of this patch into the inside-out configuration and changing the bath solution to the high-K+ pipette solution, the estimated single-channel conductance was 210 pS, as previously reported (8).
Thus, we reproduced their model (Additional files 1, 2), examined the final configurations of cell-sorting, and confirmed that the "inside and outside" configuration was not attained under the "specific adhesive relationship" with the DAH model (Additional File 3).
Although the Hm preference did not seem to contribute to the "inside and outside" configuration under the above-mentioned conditions, "inside and outside" cell sorting occurred when one cell type had an Hm preference and the other cell type had an Htr preference (Fig. 8c e).
However, when both homophilic adhesions are stronger than the heterophilic adhesion, which corresponds to the "specific adhesive relationship", the DAH model predicts that the "inside and outside" configuration of cell-sorting does not occur.
When the DRAGGING TIME of the light cell type was ≤ 10 and that of the dark cell type was equal to 30, the "inside and outside" configuration of cell-sorting occurred when the ACTIVATION THRESHOLD equaled to 0.4, the SINGLE MOVING DISTANCE equaled to 1, and the GAP PREFERENCE was Htr (Fig. 6).
These results appeared to be inconsistent with previous findings that differences in the ACTIVATION THRESHOLD caused the "inside and outside" configuration of cell-sorting [ 41].
Under these conditions, the two cell types segregated from each other within the aggregates during each simulation, but the "inside and outside" configuration – where one cell type forms a central cluster and the other cell type surrounds that cluster – was not reproducibly generated (Figs. 4, 5).
