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The next morning, after an OD600 measurement to verify overnight growth, the culture is transferred into a 25 ml glass centrifuge tube (Schott 2160114, Duran) with a 3 × 8 mm magnetic stir bar (13.1120.02, Huberlab) that is placed inside the bead bath of the light-delivery system.
At t 0 (Figure 4A) the cells were widely spread inside the bead, meaning that the immobilization process occurred as predicted.
As noted earlier, the bead pack was saturated with water (brine) followed by the oil (kerosene) injection from the top until the amount of water collected at the outlet of the core was negligible, which meant that the water trapped inside the bead pack was at irreducible water saturation.
The density of linear peptide inside the bead was kept high to provide enough material for sequencing.
Buffer flow would be sufficient to push the liposomes inside the bead net and to immobilize them.
The library was constructed to have a 10-fold reduced ligand density on the bead surface relative to the loading of linear peptides inside the bead.
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Insert the ends with the glue on them into the inside of the bead.
Pull the head pin through the hole until the flat end of the pin is pressed against the inside edge of the bead.
Heat-denaturation of the protein prior to microgel formation led to the highest encapsulation efficiency and retention, which was attributed to the formation of a cold-set protein gel inside the beads.
Morphological investigations along with thermogravimetric analysis showed that the entrapped water is either discrete water droplets of 9 10 µm in diameter or bound water with a strong adsorption to the hydrophilic nanoparticles inside the beads.
Diameter increase was caused by the yeast growth inside the beads (see Table 2).
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