However, according to the Charge Balance Rule the topogenic signals within nascent membrane proteins are interpreted in agreement with the Positive Inside Rule as influenced by the protein phospholipid environment.
These compounds seem promising to extract in a functional state membrane proteins obeying the positive inside rule.
It obeys the "positive inside" rule in which there is a preponderance of positively charged residues on the cytoplasmic side of the membrane-spanning sequence[20].
It would thereby follow the "positive inside rule" [44] and arrest the C-terminal part inside the membrane while being available for protein-protein interactions on the cytoplasmic side.
The membrane insertion is determined using Phobius prediction, but also agrees with the positive (K, R) inside rule.
Basic clusters adjacent to a transmembrane helix typically mark the cytosolic side of the bacterial inner membrane according to the "positive inside rule" [ 28, 29].
Furthermore, these intracellular loops each have several conserved positively charged arginine (R) and lysine (K) residues, in agreement with the "positive inside" rule of von Heijne [ 34, 35].
The main difference was excess of arginines and lysines before the TM in proteins with extracellular N-termini, in accordance with the 'positive inside rule' (von Heijne, 2006).
The orientations of the TM helices are guided by the positive-inside rule (von Heijne, 1992), which dictates that the more positively-charged ends of TM helices remain on the intracellular side of the membrane, which carries a negative-inside electrostatic potential.
But with one glaring difference: a no-shoes-inside rule that everyone respected, at the expense of some confused bros trying to locate their Nike high tops outside at 3AM. .
