The entire protein coding region of EGFP-pDisplay was then PCR amplified and cloned into a pEGFP backbone lacking the EGFP insert, thereby introducing a second, distinct, intracellular multiple cloning site (MCS-2).
There could also be a bias for the insertion of retrogenes in the same regions where retroelements insert given that they are known to use the same machinery [ 28] or for both of them to insert in open chromatin, inserting thereby in the same regions [ 29].
Because transformation efficiency and insert size are negatively correlated, small fragments result in remarkably decreased average insert sizes, thereby causing chromosomal walking to become difficult [ 22].
In addition, these plates can be inserted percutaneously thereby decreasing soft tissue injury and leaving a better cosmetic result.
Genomic clones that hybridized to the sd1 or rht1 probes were digested with NotI to identify clones with a greater than 4-kb insert size, thereby potentially carrying a full-length gene.
Strand selection eliminated sequences with the nucleotide analog (insert or insert + adaptor), thereby creating a sequence library with a single insert orientation.
Not only does polη replicate past thymine dimers in DNA, but — unlike the other specialized DNA polymerases — it also correctly inserts adenine residues29, thereby preventing mutations at sites of thymine dimers.
Using this strategy, one end of all inserts was identified thereby enabling the relative positions of all fragments to be deduced.
L. 96 547, thereby inserting provisions setting forth authorization of appropriations for fiscal year ending Sept. 30, 1981.
L. 104 294, § 201(1)(B), inserted dash after "thereby obtains", redesignated remainder of par.
