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The inserts from a total of 58 clones obtained from five carriage water samples were sequenced and shown to be between 103 and 808 bases in length.
The coding region of full-length ErbB4 cDNA was originally identified from sequencing of plasmids containing ErbB4 inserts from a human MDA-MB-453 breast cancer cell line and pEV7-HER4, fetalfetal human brain tissue [9].
Sequencing data were produced following a chromosome map-based approach and classical shotgun strategy completed by end sequencing of inserts from a miniBAC library.
We next ligated an LPL exon 8-specific PCR product generated from the patients DNA into the plasmid pCR2.1, transformed bacteria with the ligation product and sequenced the plasmid inserts from a total of 21 independent clones.
For the generation of probes for spotting, cDNA inserts from a single 384-well plate of non-redundant, fully sequenced, annotated human cDNA collection (Human Ensembl set RZPD1.1) were employed- (BenKahla et al., unpublished) and a second partial plate consisting of control genes and empty wells to be used for data normalisation were amplified by PCR in a 384-well format.
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Inserted in my copy of a Sunday newspaper this past June was a glossy four-page color insert from a local high school we can call CPS.
The full-length EGFR promoter inserted 5' from a luciferase reporter [ 9] was a gift from Drs. Benjamin Purow and AC Johnson.
But if the screw is inserted from a more proximal direction to Stoppa incision, this zone would be relative safe.
A cardiac catheter, with a latex saccule at the tip, was inserted from a cut in the left auricle of the left atrium to the left ventricle.
The Sfi1 insert from a Pt cDNA clone, PT0313.J16.C21 (GenBank accession GR497566; [ 32]) was labeled with αP-dCTP using a random primer labeling kit (GE Heathcare, Pittsburg, PA).
Antegrade nailing was performed with 2 K-wires (1.6 2 mm) inserted from a skin incision located 1 cm distal to the tuberosity of the deltoid muscle.
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