PCR was performed, with primer sequences generated against the genomic regions flanking the insert and a standard primer for the 3' end of the insertion sequence, to obtain homozygous insertion lines (Table S1).
A step towards the aforementioned goals is the polymer membrane functionalization through covalent bonding of chemical anchors or insertion of proteins/peptides, to obtain specific properties like recognition, catalytic activity and facilitated diffusion, mimicking the complexity of a biological membrane.
This result highlights the need for targeted gene insertion in order to obtain strains that are accurately comparable concerning heterologous gene expression levels.
Since probe position is crucial to obtaining an accurate measurement of aortic blood flow, this device is operator-dependent and studies have shown that 10-12 insertions are required to obtain accurate measurements [ 22] with an intra- and inter-observer variability of 8-12% [ 23].
It is foreseen to use, among other insertion devices, superconducting cold bore short period undulators as insertion devices in order to obtain undulator radiation with short wavelengths.
To obtain insertion alleles in AML1 and other AML gene family members, we screened the insertion allele populations at the University of Wisconsin Arabidopsis Knockout facility [ 25], obtaining alleles harboring insertions in AML1, AML3, AML4 and AML5 (Fig. 3).
The rapid thermocouple insertion method was used to obtain the flame temperature profiles.
The laser extinction measurement method and the rapid thermocouple insertion technique are used to obtain soot volume fraction profiles and temperature profiles, respectively.
GEKU-base also provides information on how to obtain insertion lines.
