Sentence examples for insertion to create from inspiring English sources

were transfected (Amaxa nucleofection, Amaxa Inc., Gaithersburg, MD) with plasmids containing NS5A sequences or with control sequences including the vector control expressing GFP, or with a vector that contains NS5A sequences with a single base insertion to create a frameshift mutation as previously described [33].

Although the study clearly shows the capacity of insertions to create protein-coding level changes specific to the human genome, the expected number of de novo genes is low.

In addition, we generated a precise excision of the Regg1 P-element insertion, Regg11c, to create an isogenic wild-type control line of the Regg1, egr1 and egr3 alleles.

These approaches can be used to map regions in a protein that are permissive for GFP insertion or to create novel biosensors.

We developed and tested a device to manipulate the axial position of the tibial anterior cruciate ligament (ACL) insertion in vitro to create a potential animal model that could simulate both ACL insufficiency and 'optimal' ACL reconstruction.

This insertion was shown to create an alternative splice donor site.

The frequency of incorporating the targeted mutation remains efficient at least as far as 460 bp from the insertion site meaning that a single insertion can be used to create many different mutants.

Conventional dynamic hip screw (DHS) insertion instruments are used to create a fusion tunnel traversing the joint from posterior to anterior.

In human, this type of insertion has been shown to create new non-conserved polyadenylation signals [ 39], influencing the level of gene expression [ 40].

We conclude that ssODNs with short (>60 nt) homology arms can be used to create insertions (largest tested: 66 nt) at or near Cas9 sites without also causing random insertions in the genome or mutations in predicted off-target sites.

It was a silk, wine-coloured gown with 310 pleated insertions, which were used to create the skirt and sleeves.