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Nokia now does insertion testing by hand; Robostan sits inside a glass case like a museum piece.
A man called Stan used to do "insertion testing" – he would plug charger cables in and out, or slide Sim cards into their slots.
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Because most P insertions tested were predicted to generate overexpression phenotypes (GS and NP lines), loss-of-function alleles of candidate genes were also checked for interaction whenever possible.
Hence, 2.5% (75/2,940) of all insertions tested for semi-lethality met the criteria for semi-lethality.
The P-1155 transgene transgene induced repression of the three PBoL insertions tested (Table 1).
Finally, the reciprocal cross was performed, and no repression was detected with any of the three PBoL insertions tested.
Similarly, of five euchromatic P-lacZ insertions, and three pericentromeric chromosome insertions tested, located on chromosomes X, 2 and 3, none repressed m2a, w1a or A7.6B (Table S1).
When P-1152 females were crossed with males carrying any one of the three PBoL insertions tested, G1 females showed strong lacZ silencing in all cases.
Scoring egg chambers at stages 9-10 allowedetectionof of lacZ expression in all (but one) egg chamber among more than 4700 egg chambers assayed for the three PBoL insertions tested.
Here, we have seen that Blackbeard and Surcouf insertions occurred at least a century ago and that both (as well as all other insertions tested; data not shown) have remained in a heterozygous state until now, in accordance with rare or absent meiotic cycles and only limited crossing overs between chromosome pairs in P. tricornutum.
Electrode insertion tests were performed in a transparent acrylic model of the human cochlea.
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