The sheep PCL had a wide posterior tibial insertion such that it almost looked as if it had two insertion sites.
Furthermore, a "minimum-possible-volume" shunt was connected between carotid artery and jugular vein, using a modified apparatus with a single T-connector for probe insertion (such that the distance from artery to probe was approximately 5 cm and the total shunt length 10-15 cm).
One concern with subcutaneous interstitial fluid probes is the tissue trauma created during insertion, such that measurements may be less accurate for several hours after insertion.
Our observation that proteins involved in membrane insertion, such as Mps2, Bbp1, and Ndc1, also accumulate at the new SPB early in duplication suggests that SPB assembly and NE insertion are coupled events during SPB formation in wild-type cells.
The angle of dart insertion was such that the umbrella tip engaged the pterygiophores immediately underneath the dorsal fin, thus reducing the possibility of premature release.
The genes encoding ribonuclease H and transposase, which are predicted to be able to target some insertions, such as group I introns that can be stably integrated into the genome following the reverse splicing reaction [ 49], belong to lineage-specific multigene families in E. pusillum (Table 2).
A model that can explain complex insertions such as these involves abnormal template switching as a part of reverse transcription.
It is possible that large insertions, such as whole-domain insertions, have arisen in the proteins considered in our dataset and are actually due to large gene insertions and not pointing to subtle functional changes brought about by small length variations.
Thus, pieces of retrotransposons that are separated by large insertions (such as insertions of other TEs) can be connected together in the genomic sequence.
This number of insertion mutants is such that, in a genome of ~4.6 × 106 bp [ 30], a marker should occur, on average, every ~3600 bp.
Based on the definitions above, observe that in the special case of an insertion in T, such that t i +1… t j −1 are all aligned to ϕ and f(t i ) and f(t j ) are consecutive nucleotides in G, G′, S and S′, the penalty is zero.
