To this end, plasmid-based approaches (Nakazawa et al., 1999), genomic insertion of resistance markers, and induction of antibiotic resistance using mutagenic agents (Putrament & Baranowska, 1973; Putrament et al., 1978) have been described.
It has been determined that two useful phenotypes segregate with the insertion: increased resistance to organophosphate pesticides (Aminetzach et al. 2005) and resistance to sigma viral infection (Magwire et al. 2011).
A gene-trap insertional mutagenesis screen produced one insertion that conferred resistance to sodium arsenite.
Deletion alleles containing a kanamycin resistance insertion (KmR) were transduced from the Keio collection [41] into the wild-type strain by P1 phage transduction (Table 1).
Deletion alleles containing a kanamycin resistance insertion (KmR) were transduced from the Keio collection [37], obtained from the Coli Genetic Stock Center Yale Universityy), into the wild-type strain by P1 phage transduction.
After confirming proper insertion of the resistance cassette by colony PCR, 80mer DNA fragments derived from oligonucleotides are electroporated in the mutant strain expressing the λ Red recombinase system.
To rule out that the increased import length was caused by polar effects of the insertion of the resistance cassette on genes downstream of mutY, we next performed a functional complementation experiment by introducing into the chromosome of the mutY mutant an intact copy of mutY that was under the control of the strong urease promoter [19].
SAP027A has an insertion encoding antiseptic resistance, and SAP053A has an additional predicted multidrug efflux protein.
In particular, unlike in most strains where comM is interrupted by an insertion of a resistance island (AbaR), in strain Ab A118 it is uninterrupted.
If a cell line is genetically modified (including methods used to achieve immortality when relevant), it is essential to describe the process used, including details of sequences, mode of insertion and antibiotic resistance markers.
