In the subscapularis, three participants had tears: one partial tear at the insertion (intrasubstance, superior portion), one full thickness tear in the tendon (superior portion), and one complete tear at the insertion.
For early detection of gene insertion, a small portion of leaf tissue (1 × 1 mm) was excised from small seedlings after 2 months recovery from the 3rd round hygromycin selection.
The mutation the insertion of a portion of DNA that can "jump" to a new location within a genome occurs within cortex, a gene known to affect cell division and egg development in fruit flies.
Systematic locus IDs will be assigned to all genes, including those that are known to have been introduced into the nuclear genome via an insertion of a portion of an organellar genome (plastid and/or mitochondria), recognizing that such genes will often turn out to be non-functional or pseudogenes.
This haplotype resulted in insertion of a portion of intron 5 into the coding sequence or deletion of exon 6, and generated premature stop codons.
We interpret this result as epitope insertion in this portion of the protein causing improper maturation and processing of the protein (retention in the endoplasmic reticulum), preventing functional insertion in the membrane as determined by the absence of an immunofluorescence signal in transfected cells (data not shown) and ultimately leading to absence of detectable transport.
At the ventral surface of the brain the lesion resulted in the insertion of a portion of the olfactory tubercle/lateral olfactory tract (white arrow in Figure 7D and E) into the piriform cortex (black arrows; compare to Figure 7G, showing the same region from a control rat).
However, it is clear that the gene rich and highly recombining environment of the euchromatin makes insertions in this portion of the genome substantially more harmful.
Furthermore, numerous cryptic splicing sites in the region surrounding this mutation obtained a high score by SSPPs analysis and were expected to cause the insertion of different portions of intron 13.
The 01640 P insertion is within the portion of exon 3 that is uniquely spliced into isoform I.
