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Given the location of this insertion, outside of the proofreading NTD, we surmise that it either disrupts the ability of ε to bind to α or results in an unstable protein.
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There is one clear-cut example of cassette insertion outside an integron emerging from the analysis of the DAT722 array.
The latter scenario of cassette insertion outside an integron context has been previously documented for gene cassettes generally associated with class 1 integrons [ 3].
In gun4-II the plasmid insertion outside the GUN4 gene has caused a genetic rearrangement of the GUN4 gene that prevented gene expression.
Factors associated with its development include IVH, insertion outside the operating theatre and EVD placement days.
This has inhibited progress in understanding retrotransposon insertion dynamics outside of a few well-studied species.
The enrichment of miRNAs loci in proximity to CIS inserts is higher than the enrichment of miRNAs around non-clustered retroviral insertions located outside of CISs.
Both the N-terminal insertion and the proline-rich wrist region insertion evolved iteratively (shorter insertions are present outside of the eutherians; Figs. 2A and 2C) and the homodimerization potential of the α-subunit appears to have been progressively reduced as the insertions lengthened.
The remaining 13 fluorescent proteins resulted from <EYOR> insertions outside the Prestin coding sequence, most of them being clustered just downstream of the CMV promoter.
Esmeraldo shows additional insertions and deletions outside of areas edited in other species in ND5, MURF1, and MURF2, while CL Brener has a distinct insertion in MURF2.
All insertions were located outside of the predicted eight-stranded antiparallel beta-barrel core structure (Fig. 4).
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