Sentence examples for insertion orientation from inspiring English sources
The insertion, orientation, and expression of inserts were determined by reverse transcription polymerase chain reaction (RT-PCR; data not shown).
Analysis of individual clones from this gene-disruption library, as well as model experiments using defined cDNA clone targets showed that in most of the resulting tagged plasmids the transposon had inserted into the moss cDNA, with no apparent preference for target sequence or insertion orientation (Fig. 2).
In real operation, by matching the guide template to patient's back skin, cement needles' insertion orientation and depth were easily established.
Therefore, the loop architecture and insertion orientation are distinctive, with in vitro splicing data indicating that a GNRA tetraloop is incompatible at this position.
The insertion orientation of the 3.0 kb HapII fragment was established by digestion with ScaI.
This tendency has been observed for transposable elements, which are believed to have different effects on gene function depending on their insertion orientation in gene bodies [ 58, 59].
However, the intensity of the bands in the ladder pattern changed subtly upon insertion of either DNA insert orientations.
Whereas these Bov-A2 insertions differ in the genomic location of insertion site, orientation and number of inserted elements, they share a common feature: the entrance site in both cases employed short palindromes that can form hairpin secondary structures.
Following PCR screening for correct insertion and orientation, the kanamycin resistance cassette was introduced into strain SL1344 by bacteriophage P22 transduction using standard protocols.
Moreover, palmitate groups are important for determining the interaction of H-Ras with specific membrane microdomains, its membrane insertion and orientation and its presence in internal membranes, which in turn may influence the engagement of various signaling pathways [8], [9].
