We also tested the effect of insertions by mapping D. melanogaster reads containing a single insertion of variable size (3, 6, 9% of the read length) in a random position.
Some of them, such as those of synaptotagmin or phospholipase Cβ, bind to membrane surface, while others, such as the C2 domain of phospholipase A2, invade the membrane by insertion of variable C2 domain loops [ 61].
Formation of C5b-9 begins with the binding of C5b6 to the plasma membrane of the target cell, with subsequent recruitment of complement proteins C7 and C8, and culminating in insertion of variable numbers of complement C9 proteins to form the lytic pore.
Sequence alignments show that there are approximately 130 conserved amino acids within BAH domains although some contain large insertions of variable length.
The fact that the domain shows insertions of variable length is not in contradiction with a stable fold [ 58]; even more, small folds, such as Ubiquitin [ 54], Immunoglobulin [ 59] or SBDS [ 60], share a high degree of structural conservation in a scenario of high sequence divergence and long loop insertions.
The phylogenetically correct residuals used in the figures were taken from the PGLM model following the stepwise insertion of prior variables in the order listed in Table 1.
Table 2 shows the various stages of the insertion of the variables, according to the proposed hierarchical model.
It controls for trend and seasonal variation and allows for the insertion of input variables to check and evaluate the effects of interventions: cinacalcet availability in this case.
In the present study's case, the order of insertion of the variables in the model included: predisposing factors, enabling factors, self-reported level of disease, level of disease evaluated by the healthcare professional, and the characterization of the healthcare service.
Unique restriction sites were introduced to allow for insertion of the variable regions of the heavy and light chains in frame with the constant regions.
