Sentence examples for insertion numbers from inspiring English sources
Chr3 refers to chromosome 3. (B) Southern blot analysis of the T-DNA insertion numbers in Col-0 and sfar4 mutants.
A Southern blot (see [35]), on the other hand, will estimate total insertion numbers.
Ten families were identified, with insertion numbers ranging from an single to 8 element insertions.
Determining T-DNA insertion numbers by Southern blotting showed that approximately 50% of the transgenic lines had a single-copy insertion.
To measure the insertion numbers per cell, cells from the original prescreened HeLa library were diluted and plated in a 96-well plate at average one cell per well.
The high frequency of HTs, combined with the transcriptional activity and low insertion numbers of Gypsy and Micropia retroelements in the Zaprionus species subgenus indicates that these elements remain at the first or second steps of the evolutionary life history of the TEs, i.e. the invasion or the genomic spread period.
y -axis, the time it takes a male to insert his spicules, x -axis, insertion number.
This includes the use of flow cytometry (for ploidy analysis), diagnostic PCRs (to confirm completeness of the insert), qRT-PCR (for gene expression analysis) and southern blotting (for detection of insertion number).
Insertion efficiency is defined as the actual channel number per vesicle determined by FCS divided by the theoretical insertion number, for which we assumed all PAP channels added to the reconstitution mixture were embedded into the vesicle bilayers (Fig. 2f, see Supplementary Methods).
Southern blotting indicated a low T-DNA insertion number in the orchid genome of the transformants.
For each case, several data were recorded, concerning both the patient (gender, age, BMI, primary diagnosis) and the venous access (insertion-related complications, success at insertion, number of attempts before insertion, length of the procedure, insertion site, diameter and depth of the vessel, duration of the access, late complications).
