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∼11,000 clones from the M. smegmatis insertion mutant library were replicated onto plates with (Rif) or without (No Rif) rifampicin (step 1).
Furthermore, the extent of root growth inhibition by high-B stress was lower in brm-3, a T-DNA insertion mutant of BRM (Fig. 6a), than in the wild type (Fig. 7b).
We screened a transposon-generated E. coli insertion mutant library, using a qualitative PZAase assay.
Genotyping of the T-DNA insertion mutant was performed by Southern and PCR analysis.
A T-DNA insertion mutant of OsABF2 showed increased sensitivity to abiotic stresses compared to wild-type plants.
The 129B08/nced3 mutant was a T-DNA insertion mutant requested from the Nottingham Arabidopsis Stock Center.
A functional homologue of SOS1 in rice, OsSOS1, had been isolated and a T-DNA insertion mutant of OsSOS1 exhibited a salt sensitivity.
Most, if not all, of the FST information from all insertion mutant population is available in rice browsers RiceGE and OygenesDB.
Another important issue in the insertion mutant resource is the low tagging efficiency; that is, the relationship between genotype (integrated/affected gene) and phenotype is very low.
With this linear and exponential TAIL-PCR (LETAIL-PCR), we successfully obtained products larger than 2 kb from Tn5 transposon insertion mutant strains within 3 h.
(v) Fiber knob insertion mutant.
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