Exact(3)
Following membrane insertion, most of the surface of an integral membrane protein is in contact with the membrane's hydrophobic interior.
The data show that although the structure of MscS may be perturbed by Trp insertion, most of the mutants form stable heptamers that can be purified and reconstituted into lipid bilayers.
For Mn2+-dependent nucleotide insertion, most of the variants (including the Δ1 25 variant) showed the kcat/ Km values similar to those of wild-type, whereas the R96G variant displayed 29- to 41-fold reduction in kcat/ Km compared to wild-type (Table 5).
Similar(57)
These insertions are composed of several TE insertions, most of them similar to elements previously characterized in D. buzzatii [38], [60].
Of these 1000 insertions, most of them (90%) were into the single copy regions, and more than 70% were located within 5 kb of transcribed DNA.
The analysis of the mined copies revealed that some reference copies harbor insertions, most of them smaller than 200 bp, and a few larger than 200 bp.
Moreover, we identified and validated large insertions, most of which range from 150 bps to 1.2 kb in length in the CB4856 strain.
Within the ABD we have identified sequence insertions, most of which uniquely characterize sequences from Group III (HSPA4, HSPH and HYOU1, see Figure 6).
This analysis revealed that some reference copies harbor insertions, most of them smaller than 200 bp, and a few larger than 200 bp.
Second, we identified an impressive rate of rDNA cluster change (deletions and insertions), most of which somewhat surprisingly occurred in the subgenus Mus, an evolutionary clade in which all species share the same chromosomal complement.
Alignment of NELoc-1 and -3 and flanking sequences with members of this family reveal a shared structure and site of insertion, most closely resembling pCP8533etx, suggesting that the NELoc-1 and -3 plasmids are genuine members of this growing plasmid family.
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