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None of the patients was aware of the insertion method used.
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To compare a new insertion method using a suture with the standard forceps-assisted insertion of the lamellar corneal graft in Descemet stripping automated endothelial keratoplasty (DSAEK).
To compare a new insertion method using an EndoGlide (Angiotech/Network Medical Products) with the standard Busin glide (Moria USA) assisted insertion of the posterior lamellar corneal graft in Descemet stripping endothelial keratoplasty (DSEK).
The insertion method uses a pre-equilibrated lipid bilayer with a hole of an appropriate size into which a protein is inserted.
The methods used to generate the decoy sets include Monte Carlo optimisation with simulated annealing [18], random search of conformational space with subsequent local minimization [10], molecular dynamic trajectories [19] [21], the graph theoretic algorithm [22] and a fragment insertion method using Bayesian scoring functions [23].
Slesak 2003 measured doctors' and nurses' perceptions of the feasibility of each insertion method using a Likert-like scale.
Thus, we established a targeted insertion method using the BGM vector system with homologous recombination using several antibiotic resistance genes and the cI gene without leaving any trace of selection markers or site-specific recombination sites, such as loxP and FRT, in the target sequence [ 5, 6].
In addition, this model is not directly comparable with the routine stent insertion methods used in the clinic.
Knock-ins have been primarily generated through efficient selection schemes based on the earlier MosI-mediated single-copy transgene insertion methods using genetic markers such as unc-119 (Dickinson et al. 2013), drug resistance markers (Chen et al. 2013b), or fluorescence (Tzur et al. 2013).
We estimated the recombination rate at each TE insertion site method using a method previously developed for the D. melanogaster genome [ 54].
The insertion method was used to place the peptide system into the bilayer (~10.0 Å from the edge surface).
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