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Compensation for degradation of mechanical properties of matrix (due to inclusion insertion) is obtained by embedding fibers into the mixture.
Starting from the 5' or 3' sequence flanking the transgenic insert, usually already known as part of the molecular characterization of the event, the sequence of the site of insertion is obtained in a non-GM genotype using currently available strategies.
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Smaller error with preloading-based insertion than with normal insertion was obtained in all experiments [25]-[28 [25]-[28
Homozygous mutants of OsBAK1 without T-DNA insertion were obtained from T1 generation.
Homozygous transgenic lines that contain YZ54 and YZ55, respectively, with a single T-DNA insertion were obtained by hygromycin selection.
Visual analogue pain scores 0-1000 mm) at catheter insertion were obtained on all but two women (96%% response rate).
Effective ventilation after mask insertion was obtained in 97% (65/67); in the two unsuccessful cases, one was hanged and one had a literalised larynx (these data were not collected in four patients because of a recording problem).
Outcomes following catheter insertion were obtained including technical success, discharge from hospital on the day of procedure, catheter leaks and migration, time to first use and exit site infection and peritonitis rates.
It lists whether the insertion is located in a gene desert (a gene-free region larger than 500 kb), intergenic (less than 500 kb-long), intronic or exonic region, specifies the orientation of the reporter gene, and the parental insertion line from which the insertion was obtained.
Excision of the plasmid from the chromosomal site of insertion was obtained by repeated passage of a mutant clone on agar plates without erythromycin at 30°C, and the sites of plasmid insertion were determined by sequencing of plasmid-borne fragments derived from PCR with the primer pair LSV3 and LSV-4380rev [ 12].
Formalin fixed paraffin embedded placental tissue samples (1 sample per placenta, collected between the rim and point of chord insertion) were obtained from the tissue bank facility of the UOEH Pathology Department with IRB approval (full term pregnancy, n = 10; pre-term, n = 10; preeclampsia, n = 10; and IUGR, n = 10).
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