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The remarkable efficiency of this intron represents the upper end of intron targeting efficiency [ 19]; i.e. 100% insertion efficiency for all colonies screened with 1 in 24 colonies showing an intron insertion into three independent chromosomal insertion targets.
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We have examined the impact of a transposon insertion into one of these genes (EXPB1, the most abundant isoform of Zea m 1) on the production of Zea m 1 protein, pollen viability, and pollen tube growth, both in vitro and in vivo.
Two second-order resonances corresponding to an AA′XX′X′′X′′′ spin system are expected for the isomer of 42 2+ formed via formal insertion into two opposing P P bonds of P4.
We report here for the first time the complete in situ Raman study of lithium insertion and de-insertion into three variously nano-sized TiO2 anatase powders (Prolabo, ca. 80 nm, AK1, ca. 15 nm and MTi5 ca. 8 nm), of which AK1 and MTi5 show superior capacity and cyclability.
We divide insertions into three mutually exclusive types: (i) novel insertions in the donor genome that have no match in the reference, (ii) duplicated insertions, which are found at two or more sites in the donor and a strict subset of those in the reference and (iii) transpositions, which are sequences in the reference that moved to a different site in the donor.
These errors can arise because of unique insertions into one or more regions of sequences, or through some more complex evolutionary process leading to proteins that do not align easily by sequence alone.
The insertion sites and orientations were confirmed for seven enhancer trap lines with insertions into five NBS-LRR-encoding genes and two TIR-NBS-encoding genes (Table 5, Figures 2 and 3).
We wish to see whether the predictions of our model are borne out by genomic data and so for eighteen genomes we aggregate retroviral insertions into two groups for easy display and analysis: those on the allosome (X or Z) and those on the autosome.
The four non-oxidative PPP genes TAL1, TKL1, RKI1 and RPE1 were upregulated by insertion into the two alleles of the GRE3 locus (Additional file 1: Fig. S1d).
In addition, through simulations, we identified the amino acids that are most responsible for the adsorption onto or insertion into the two model membranes.
SEC24A-deficient mice were generated from an ES cell line carrying a gene trap insertion into intron two of Sec24a.
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