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In order to assess the role of Hsp90α in vivo, a mutant mouse line was established from an embryonal stem cell clone carrying a gene trap insertion in the last intron of the Hsp90aa1 gene (Fig. 1A).
For comparison, we studied another DGAT1 mutant, which we designated A7, which is a homozygous SALK line (Salk 039456) with a T-DNA insertion in the last exon of the same DGAT1 gene (At2g19450).
Genomic PCR and sequencing revealed a full-length intracisternal A-particle (IAP) proviral insertion in the last intron, present in HeJ but absent from B6 and FeJ strains (Fig. 2, Supplementary Material, Fig. S1).
wrky25-1 (Salk_136966) contains a T-DNA inserthen in the promoter region while wrky25-2 (Sail 754_A03) contains a T-DNA insertion in the last intron of the WRKY25 gene.
Two lines obtained from T-DNA mutant collections were characterised by sequencing from both T-DNA borders: SALK_102345, an insertion in the last exon of REV (At5g60690) upstream of the termination codon, and GABI-KAT line GK_302G09, an insertion affecting the second exon of CUC3 (At1g76420).
All of the members of Group II contain one more exon and a phase-2 intron at the sixth position, indicating that there was an intron insertion in the last exon, which contributed to the generation of Group II and expansion of the VvCDPK family.
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Genes that had transposon insertions in the last 5% of the gene (3' end) were filtered out since they may likely to generate active product and the remaining reads were mapped against the B. fragilis 638R genome [ 14].
The different transcripts differ in three regions: in the first, four sequences have a 122 nt insertion, in the second, four sequences have a 94 nt insertion and in the last, four sequences have a 88 nt insertion.
These results therefore suggest that to create more effective questionnaires the most important information should be collected in the first fields, the number of insertion-fields per insertion-sheet should be reduced and combo-boxes or text-boxes should be used instead of fields with direct numerical insertion (especially in the last part of the questionnaire).
This gene-trap insertion is in the last coding exon of the Sema5A gene, generating a deletion mutant lacking the C-terminal portion of the cytoplasmic domain, a region of Sema5A that we find is not necessary for the regulation of dendritic spine density.
Candidaemia in patients who had undergone major surgical procedures in the last 30 days or prosthesis insertion in the last year was considered to be nosocomial.
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