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The z3 2 and z3 3 mutants have a T-DNA insertion in the first exon, but in different locations.
Interestingly, an additional allele was also uncovered (named the B-allele) that contained a 45-base pair insertion in the first intron.
The remaining 104 germplasms contained a novel allele (termed the B-allele) (Additional file 6), which had a 45-bp insertion in the first intron and did not exhibit the C165A mutation (Takano-Kai et al. 2013).
Aif gene impairment is due to an ecotropic proviral insertion in the first intron of the gene, as shown by Klein et al. [14].
Silence insertion in the first or second segment gives rise to the characteristic tri-segmented structure of the Japanese quail's crow (Fig. 1) [45].
These mice have an 80% reduction in AIF protein due to a proviral insertion in the first intron of the AIF gene.
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We also identified a T-DNA insertional mutant of this gene (mtd1 1), which has a T-DNA insertion in the sixth exon (Fig. 6b).
We identified jmj13, a T-DNA insertion in the second intron of JMJ13 (GAt5g46910, At5g46910) (Supplementary Fig. 6a 35.
An insertion line NG6411 was isolated with a Tos17 insertion in the second exon of OsINV3 (OsINV3106100).
This osphyA-2 mutant is a null allele generated by a T-DNA insertion in the fourth exon of OsphyA.
Fig. 1 Characterization and phenotypic analysis of the mutants (KO). a OsINV3 gene in 5′-3' direction with the Tos17 insertion in the second exon in reverse orientation.
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