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To compare three clinically cementless total hip stems of fundamentally different design, the strain distribution before and after insertion in human cadaver femora was measured in vitro.
In our study, we chose to insert the iVPR system into the AAVS1 locus, since it has been used as a 'safe habor' for transgene insertion in human stem cell systems (Dekelver et al., 2010).
Frequency of the AluYb8 insertion in human populations differed based on their geographic affiliation (Table 2, Supp. Fig. S3).
Inverse PCR analyses revealed that 16 clones contained PB transposon integration sites (Supplementary information, Figure S5A) at an efficiency similar to PB insertion in human ESCs.
The uniqueness of the WNK1 AluYb8 insertion in human was supported by a negative result of the BLAST search among available genome sequences.
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On the assumption that each of these 1,708 loci is indeed fixed for the insertion in humans, the rate of ME-Scan false negative results ranges from 8%to25%5%.
Ivanovski et al. [ 19] reported that the transcriptional profile of osseointegration after implant insertion in humans predominantly involved genes that are related to the immune response and extracellular matrix (ECM) formation.
Hancks, D. C. & Kazazian, H. H. Roles for retrotransposon insertions in human disease.
Currently, one array-based approach has been conducted to detect retrotransposon insertions in human disease.
Importantly, the number of new L1 insertions in human colorectal tumors was not correlated with the degree of hypomethylation of L1 promoters [ 21].
Applying our method to the genotyping of L1 insertions in human samples, we show that it is as accurate as "gold standard" site-specific PCR assays.
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