The resulting plasmid, pCMV-Tag4A-3X-Flag, conthens the insertion DYKDHDGDYKDHDI in front of the existing Flag-tag DYKDDDDK, thereby generating the triple Flag epitope.
The insertion in AKIN10 in front of the last exon was confirmed by PCR, but the second insertion in IMS2 could not be detected and was thus assumed not to be present.
In addition, insertion of a terminator in front of argF was an efficient strategy for improving l-ornithine production, by slightly relieving the growth disturbance, by blocking l-ornithine degradation.
Interestingly, compared with the control strain with argF deletion, l-ornithine production titer was improved by 29.5% after insertion of the rrnB terminator in front of argF.
The opening for insertion of the body is in front of this figure.
Based on 3' to 5' directionality, the Snf2 insertions should contact the DNA downstream (in front of, as for the location of helicase "pins") of the contacts conserved among SF2-type helicases.
The first dorsal fin is triangular with a rounded apex, originating in front of the pelvic fin insertions.
It is approximately 800 residues longer than other MAGE proteins due to the insertion of a large number of short repetitive sequences in front of the MAGE-homologous sequence.
The tub cluster was inserted into the chromosome of P. putida using the MycoMar transposon and was expressed relying on the native promoter(s) and by additional insertion of the P tet promoter from transposon Tn10 in front of tubCDEF.
Interestingly, even if the nolR gene is disrupted by a single base insertion in strain Rm1021 [ 46], the NolR binding sites in front of nodD1, nodD2 and nodA are maintained, suggesting that the inactivation may be relatively recent.
A gene variant called A vy is a dominant mutation that is caused by the insertion of a DNA sequence known as an intracisternal A-particle (IAP) retrotransposon in front of (i.e., upstream of) the Agouti gene.
