Sentence examples for insertion in front from inspiring English sources
We utilized an EP-element insertion in front of the Gdh gene to activate expression of Gdh.
The setup acquisition dynamic range was extended up to eight orders of magnitude with neutral optical filter wheel FW insertion in front of the PD.
It is likely a major enzyme for such bypass in mammalian cells as it can perform both insertion in front of the damage and extension of the misincorporated nucleotide [ 9, 10].
The promoter deletion constructs for the SIX1 and SIX3/SIX5 promoters were made by PCR amplification sequences of the sequences flanking the part of the promoter that was to be deleted for homologous recombination, and their insertion in front of and behind the hygromycin resistance gene in the vector pRW2h (see below).
The resulting plasmid, pCMV-Tag4A-3X-Flag, conthens the insertion DYKDHDGDYKDHDI in front of the existing Flag-tag DYKDDDDK, thereby generating the triple Flag epitope.
The insertion in AKIN10 in front of the last exon was confirmed by PCR, but the second insertion in IMS2 could not be detected and was thus assumed not to be present.
In addition, insertion of a terminator in front of argF was an efficient strategy for improving l-ornithine production, by slightly relieving the growth disturbance, by blocking l-ornithine degradation.
Moreover, for insertion of the terminator in front of argF, terminators derived from a previous study (Chen et al. 2013) were added between the upstream and downstream sequences by overlap PCR and then cloned into plasmid pK18mobsacB.
As expected, we couldn't detect the transcripts of the KCBP after the T-DNA insertion, but we indeed detected weaker transcription of partial KCBP in front of the insertion site (please see the new Figure 1 figure supplement 1).
Interestingly, compared with the control strain with argF deletion, l-ornithine production titer was improved by 29.5% after insertion of the rrnB terminator in front of argF.
The opening for insertion of the body is in front of this figure.
