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The reactions at either end of each locus were designed to extend into the flanking sequences to verify the site of insertion (Figures S6, S7, and S8).
Similar(58)
This paper focus on investigation to test the probe with round concave shape (termed 'concave' probe) and to evalauate preloading concept with concave probe to enhance accurate needle insertion (Figure 1).
This analysis also revealed a 265 bp deletion downstream of the erythromycin insertion (Figure 2).
No functional transcript could be detected in plants homozygous for the insertion (Figure 5).
Acute airway obstruction with asphyxia was described after esophageal stent insertion (figure 1, 2).
There was no noticeable alteration of this phenotype in flies carrying an extra UAS insertion (Figure 4B).
All five baboons had only the shorter PCR product, demonstrating the absence of the CypA insertion (Figure 2b).
As with single nucleotide deletions, insertion sites will nearly always generate either no reads or a mutant read in the vicinity of the insertion (Figure 5C).
As expected from these results, none of the M. sylvanus tested had either the CypA insertion (Figure 3b) or TRIMCyp mRNA expression (Figure 3c).
Southern blot analysis performed on the two trkH mutants and on the 4 auxotroph mutants, confirmed that they (Table 2) contained a single chromosomal transposon insertion (Figure S1).
We therefore measured K' for L-arginine in phosphorylated or non-phosphorylated recombinant nNOS in the presence of BH4 and cholesterol, as a lipid phase representative of membrane insertion (Figure 5B).
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