In contrast to B. subtilis, the removal of the insertion element led to lower transformation efficiencies (Hoffmann et al. 2010).
Removing the insertion element (and thereby restoring an active copy of comP) resulted in reduced genetic competence (Hoffmann et al. 2010), which clearly differs from B. subtilis.
We have developed a loop-mediated isothermal amplification (LAMP) assay to detect the presence of the transposase gene insertion element IS1111a of C. burnetii.
RD6 is an insertion element shown to be variably deleted in mycobacterial strains irrespective of origin [37].
Twenty-five of these were associated with differences in the presence of the mobile insertion element IS6110.
Furthermore, the insertion element IS630 found in ICEEc1 encoding the transposase TnpA is lacking in the ICE of HPI-ICEEh1.
These included SciE (ImpE family protein), SciT protein, OmpA, a serine threonine kinase protein, a phosphoprotein phosphatase, TPR repeat protein, transposase, and insertion element IS-4.
The IS6110 multicopy insertion element, the most widely used target sequence of the M. tuberculosis genome, does not allow specific identification of M. tuberculosis [27].
In the Paris strain, the insertion element is restricted to one copy each on CII and p74; these copies are maintained in the Ames strain.
A closer look at the left region suggested that an IS6100, exhibiting 100% identity to that of Mycobacterium fortuitum [16], was interrupted by a novel insertion element ISSsp1.
Strains and plant growth conditions Arabidopsis thaliana strain ET500 (La-er) was the source of Ds insertion element [19], and strain NaeAc (No-0) was the source of Ac transposase [35].
