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Insertion direction of cmx transposon was determined by sequencing with primer xj146.
The forward-viewing ability is particularly relevant to guiding minimally invasive interventions, especially those that involve needle- or laser-based procedures22,23,38,39,40,49 where it is often desirable to "look ahead" of the insertion direction.
When forming an implant cavity, it is necessary to move the drill back and forth along the insertion direction.
Constructed vectors were sequenced to confirm the correct cDNA insertion direction.
A sagittal insertion direction was used, and we avoided a medial angulation of the needle.
The insertion direction of the EmrE gene product (Multidrug resistance family) can be controlled in a model system, to show that interaction between two oppositely oriented single DMT domains is responsible for protein activity [ 25].
Similar(54)
We found that the ratios of targeted insertion with right direction were at least 50%, with 3/6 lncRNAs showing 100% targeted insertion with right direction (Table S1).
Established targeted insertion with right direction was confirmed at the genomic level with PCR (Fig. 1D).
As a small amount of puromycin proteins may be produced from CMV-puromycin selection cassette without polyA signal in cells containing targeted insertion with reverse direction, we picked out 2 5 clones for each lncRNA and examined the ratio of clones containing insertion with correct direction.
The program automatically estimated the insertion size and direction between the paired reads and discarded the inferred incorrectly mapping pairs.
In all insertions, the transcription direction is altered from that in the original position.
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