Sentence examples for insertion data from from inspiring English sources
An alternative method to generate SB transposon insertion data from unselected tissue in vivo has previously been utilized.
As a result, current methods of interpreting insertion data from mutagenesis screens do not consider the abundance of each individual site.
We averaged the insertion data from seed mice from each line to calculate the likelihood (rate per copy number) that a transposon could be mobilized from their respective concatemers (Table 1).
The T2/GT3/tTA line 6660 was used in a study of mutations induced specifically on mouse chromosome 11 and the insertion data from this line will be reported elsewhere (A.M.G., manuscript in preparation).
We retrieved insertion data for 1,522 previously described protein trap lines from the FlyTrap database [ 13].
By comparison, similar analysis of the MuLV insertion data present in the RTCGD database identified 160 CISs from 5435 insertions, 54 of these being shared between these datasets.
Finally, for the data set with deletions, the length effect and heterozygosity was calculated only from deletions and, likewise, deletions were ignored for the insertion data.
This band was absent from samples containing induced full-length OmpA without insertion and weakly detected in induced full-length OmpA with the SA-1 insertion (data not shown).
The goal of our study was to address these potential confounding factors in order to enhance functional interpretation of insertion site data from tumors.
Because the experimental conditions we used to characterize background transposition were specifically designed to match those of in vivo SB mutagenesis screens, our results are particularly relevant to the functional interpretation of insertion site data from such screens.
Current methods to analyze insertion site data from SB mutagenesis screens typically assume that all TA dinucleotides in the genome outside of the local chromosome have an equal chance of being detected as insertion sites in the absence of selective pressure.
