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Generally, fixation devices distant to the joint line (i.e., buttons, staples, washers or post-screws) fail to reconstruct the complex nature of the native tibial or femoral ACL insertion close to the joint surface.
Also the lid insertion close to the active site and the oxyanion hole has been characterized.
In this study we describe the isolation of a mutant, named T192, with a transposon insertion close to the 3' end of the mg192 gene encoding for P110 adhesin.
In our hands, the frequency of recombinant clones was higher than 90% for both VH and VL insertion, close to the efficiency reported for different vector systems based on Gateway technology.
A comprehensive comparison of large and small subunit amino acid sequences by Badger and Bek [14] revealed a six-amino acid insertion close to the N-terminus of noncarboxysomal CbbS proteins that is not present in carboxysomal small subunits.
This observation may suggest that a second insertion close to the EXO gene impaired development of homozygous plants.
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The "islands of speciation" that define the M and S forms may thus be extending gradually, reducing gene flow and fixing some TE insertions close to the selected islands.
Insertions close to the 5' end may also be possible if a suitable alternative initiation codon is available.
We found one mutation, G342A, that does not adversely affect the association of PA with ANTXR2-expressing cells yet increases the pH threshold of toxin insertion closer to that of an ANTXR1-bound prepore.
In addition, despite the fact that retrogenes use machinery of transposable elements, they do not follow the transposable elements genome location pattern recently described in detail [ 20]: high density in the X chromosome and consistent bias insertion close to female and male germline expressed genes.
Finally TE insertions within 200 bp of a splice donor or acceptor site have measurable but modest effects, suggesting the splicing machinery is fairly tolerant of TE insertions close to these sites.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com