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Generally, fixation devices distant to the joint line (i.e., buttons, staples, washers or post-screws) fail to reconstruct the complex nature of the native tibial or femoral ACL insertion close to the joint surface.
Also the lid insertion close to the active site and the oxyanion hole has been characterized.
In this study we describe the isolation of a mutant, named T192, with a transposon insertion close to the 3' end of the mg192 gene encoding for P110 adhesin.
In our hands, the frequency of recombinant clones was higher than 90% for both VH and VL insertion, close to the efficiency reported for different vector systems based on Gateway technology.
A comprehensive comparison of large and small subunit amino acid sequences by Badger and Bek [14] revealed a six-amino acid insertion close to the N-terminus of noncarboxysomal CbbS proteins that is not present in carboxysomal small subunits.
This observation may suggest that a second insertion close to the EXO gene impaired development of homozygous plants.
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We found one mutation, G342A, that does not adversely affect the association of PA with ANTXR2-expressing cells yet increases the pH threshold of toxin insertion closer to that of an ANTXR1-bound prepore.
The "islands of speciation" that define the M and S forms may thus be extending gradually, reducing gene flow and fixing some TE insertions close to the selected islands.
Targeting transgene insertions close to endogenous TAD boundaries may therefore represent the safest strategy to house such transgenes.
Insertions close to the 5' end may also be possible if a suitable alternative initiation codon is available.
These large decreases in expression level are most pronounced for transposable element insertions close to transcripts and the effect diminishes for more distant insertions.
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