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Blood plasma samples were taken and a gynaecological examination was done with speculum insertion, and collection of endocervical and high vaginal swabs.
The PDMS chip was designed to have an inlet and outlet port for the insertion and collection of cells, and a hydrodynamic focusing region to force the cell sample into a thin (sub-cell sized) stream down the center of the fluidic channel thereby directing all cells through the photoporation beam.
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OryGenesDB contains >245 000 flanking sequence tags of various mutagens and functional genomics data collected from both international insertion collections and the literature.
The primers were designed in Primer3 (Rozen and Skaletsky 2000) after a BLAT search to locate the insertion site in the Anolis genome database and collection of 300 bp upstream and downstream of the insertion site.
For Arabidopsis, large sets of "activation tagging populations" have been generated containing several T-DNA-based activation tagging vectors which are readily available from insertion collections and stock centers [ 19, 20].
The two hcnC mutants of PA14 were NRlib 03_2:e9 and NRlib 15_1:a4 from the transposon insertion library collection [31].
By searching the SALK Institute sequence-indexed insertion mutant collection (http://signal.salk.edu/cgi-bin/tdnaexpress) and the GABI-KAT database (http://www.gabi-kat.de/), we identified one T-DNA insertion mutant of the GCL2 gene, GABI_115D02.
By searching the Salk Institute sequence-indexed insertion mutant collection, we obtained two independent T-DNA insertional alleles for each RACK1 gene.
The A. thaliana T-DNA insertion line SALK_019917 for Fd-GOGAT1 (At5g04140) was identified in the SALK T-DNA insertion mutant collection [ 121], seeds were obtained from the European Arabidopsis Stock Centre NASC [ 122] and homozygous mutant plants (called glu1-2) were obtained.
A call has recently been launched for a shared international effort in the fast generation of an insertion line collection of the temperate japonica cultivar Kitaake (G. An, unpublished).
Funding for the SIGNAL indexed insertion mutant collection was provided by the National Science Foundation.
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