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Therefore, we tested the nucleoside diphosphate insertion activities of K65 substitution mutant RTs in a single nucleotide extension assay.
Importantly, the co-translational protein insertion pathway was solely responsible for these insertion activities since almost no glycosylation was detected during a post-translational protein insertion assay.
What, however, might have induced the declines in subpopulations of autonomous elements essential to high genome-wide rates of insertion activities?
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Thus, the active membrane insertion activity of YidC in the cell leads to only a temporary elongation arrest and a repressed expression level of YidC2, whereas the reduced YidC activity of the cell leads to a prolonged MifM arrest and consequent induction of YidC2.
In the absence of Htr, SR is inserted into the membrane with far less efficiency, and membrane insertion activity can be restored by the addition of the DNA sequence encoding the signal sequence peptide of BR [ 54].
Optimization of C2 oxygenate activity and selectivity requires a proper balance between CO dissociation activity, CO insertion activity, and hydrogenation activity.
All of the catalysts tested, except H2S-treated Os, exhibited CO insertion activity in the CO/H2/C2H4 reaction leading to C3 oxygenate formation.
It also retains the insertion activity in mammalian cells with a plasmid to chromosome transposition rate about 10-fold higher than random integration.
We show that this assay system is capable of simultaneously monitoring the integrity of the chimeric protein, its membrane insertion activity, and the ability of the transmembrane segment under study to dimerize.
Epsin proteins were initially chosen as a paradigm for insertion activity in the absence of scaffolding.
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