Sentence examples for inserting site from inspiring English sources

The site 0.5-1 cm outside and above the 3rd posterior sacral foramina will serve as the inserting site.

Therefore, the site for exogenous gene inserting should be comprehensively considered based on viral genomic characteristics, and the selected inserting site should be finally determined by experiment.

The needle is inserted at the inserting site inward and downward at a 30 45 degree angle to a depth of 50 60 mm.

After determining the inserting site, based on the amplification strategy), the Nsp2-GFP gene was amplified by overlap PCR using PRRSV infectious clone plasmid FL12 and gfp gene-contained plasmid pcDNA-EF1-GFP as the templates.

To construct GFP-PRRSV infectious clone, the inserting site for gfp gene should be selected properly so as to make gfp gene express efficiently and the GFP-PRRSV infection and replication in the host cells should not be significantly affected upon gfp gene insertion.

The specific Spe I site upstream and Xho I site downstream gfp inserting position was also considered during the inserting site determination.

The coding regions for viral nonstructural proteins, especially for the proteins with multistructural domains, are commonly selected to be the candidate inserting sites for exogenous genes.

Previously, we studied the exonization effect of a single inserted site by Ds providing 5 splice donor sites (1 for forward and 4 for reverse pattern), which may result in 5 exonized transcripts.

The second reaction with primers (0168-1/0168-2 of pD1-1/p pD1-2d-2) of inserted site verified the wild-type was replaced completely.

We attempted the 5′RACE method first because the mRNA upstream of the inserted site was expected to be trapped by the splice acceptor localized at the beginning of the gene trap vector.

However, tools to insert site-specific methylation into DNA for analyzing epigenetic functions are limited.