Sentence examples for inserting sequence from inspiring English sources

Our results (submitted) plus Shi's observations (Shi et al., 2015) revealed an unexpected contribution of this unique inserting sequence in constituting an extra-ligand binding motif for FadR regulatory protein.

The expression vector was created by inserting sequence corresponding to the (His 6 and XP tags of the Invitrogen pcDNA3.1/His C vector (Cat. no. V385-20) inthethe Kpn I site of the pCMV-Sport-βgal vector, following removal of the βgal ORF.

We selected validated SNPs from dbSNP to avoid inserting sequencing errors into our dataset.

In case of insertion, the newly inserted sequence would be composed of segments of different RUs.

The insert sequence is available at GenBank under accession number 1288449.

Unique insert sequences were identified within each genetic selection with few clones containing the same insert sequence.

The recombinant plasmid constructs were then purified and the insert sequence confirmed by PCR and sequencing.

Correct size of the inserted sequence was verified by restriction digestion analysis.

FLUC functions as the control reporter and is expressed independently of the inserted sequence.

Clones were tested for correct insert sequence and orientation using the M13 forward and reverse primers.

Sanger sequencing confirmed the insert sequence.