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We constructed an in-frame fusion of the NLS using requisite primers and PCR, and generated a mutant NLS by inserting point mutations.
By inserting point mutation into the Sec7 domain of tobacco ARF-GEF NtGNL1a, we have induced its BFA-sensitivity and thus uncovered its preferential role in the endocytosis of FM 4 64 and PIN1.
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We measured the time required from selection of the insert point until connecting an infusion line wearing rubber gloves.
In all cases, the ipsilateral medial lemniscus (ML) could be traced from the level of the inserted point to the level of over one centimeter caudally.
Target insert point was marked with green color.
Directed mutagenesis with PCR amplification, DpnI digestion, and transformation were classically performed to insert point mutations in the pCUP1 S 288 c variant.
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We divide the landmarks into two categories, namely key points (KPs) and inserted points (IPs).
I have heard from Bosnian women whose vaginas have been shredded by soldiers who inserted pointed objects and guns into them.
Furthermore, the sequences of CHR and CAT may also be aligned with the cell penetrating peptide (CPP), Penetratin, i.e. (Figure 9 insert), pointing the possibility for CHR and CAT, as new members of the family of CPPs [49].
As tools improve, we expect to see new insert points and new success stories.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com