Exact(11)
By inserting each gene separately into mammalian cells, they found their target: the gene that produces a protein called CdtB.
Inserting each PCR generated fragment one at a time into the destination vector is straightforward and ideal for small projects with few insertions.
The retroviral vectors encoding TAK1, MEKK3, TRAF6, T6Rm, and IRF7 were constructed by inserting each cDNA generated by PCR into the appropriate sites in the pMx-puro vector.
To this end, each CNS was amplified by PCR on mouse genomic DNA and chimeric constructs were generated inserting each fragment in the pGL3-basic reporter vector containing the E1b minimal promoter upstream of the firefly luciferase.
This process involved an iterative process of inserting each of the initial codes into larger categories, based on their 'semantic fit' or the ways in which they seemed to be relating to a similar idea or issue.
To express GST fusion proteins, pGEX-2TK USP17K, pGEX-2TK- USP17L, pGEX-2TK- USP17M, and pGEX-2TK USP17N were cloned by inserting each USP17 subfamily member into pGEX-2TK vector (Amersham Life Science, Uppsala, Sweren).
Similar(49)
They inserted each of their multiwalled tubes into the "nanomanipulator".
She stood at the door and watched him insert each pencil into the slot and then crank the handle.
He inserted each funnel, the wide part facing out, into the holes that he had made in the mesh netting.
Empty View-Master reels are no longer made, so photographers scrounge for old or used ones and then insert each tiny square by hand.
In the United States more than 1.5 million pulmonary artery catheters (PACs) are inserted each year.
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