The constructs contain multiple cloning sites (MCS) allowing any gene to be inserted using a range of restriction sites, with the option of a 5′ integral intron for efficient gene expression.
The tension sensor (TS) module has been inserted into a range of proteins, where it reported tension over both cadherins (E-cadherin and VE-cadherin ) and vinculin at cell-cell junctions.
The packet insert indicates a range of 900 1300 mg per 1 g vial.
When the sensitivity of 3-D cultures to BAC-induced cytotoxicity was evaluated by incubating the inserts with a range of concentrations of BAC for 60 minutes, BAC concentrations comparable to those found in commercial ocular solutions (<0.025%) showed no differences in viability relative to the saline control.
Nu for the PW-XT insert is in a range of 1.96 5.06 times while f increases around 2.06 35.68 times above the plain tube alone.
Prior to the arrival of NGS platforms, WGS on larger genomes was possible because Sanger-based sequencing platforms generated paired-end reads of 700 bp or greater and a range of insert sizes from 3 to 40 kb (Weber and Myers 1997).
The metagenomic libraries consisted of a total of 23,872 clones: 7,744 from SRF, 8,448 from SAB and 7,680 from LAB, with a range of insert sizes of 30 35 kbp.
Based on the age and diversity of pine repeats, it may even be possible to assemble the genome using a whole genome shotgun strategy based on several platforms and a range of insert sizes.
For each of the two chromosomes BACs were created randomly to provide 5× coverage by defining an average insert size (120 bands) and a range of variation (from 50 to 190 bands).
The structure of the complex is modified in some insects, Drosophila being an example, where two bHLH-orange domain genes are absent from the complex and there are seven copies of the remaining one, the Brd-class gene, mα, has been duplicated and there are a range of unrelated genes inserted in the complex [ 6, 9– 16].
