Sentence examples for inserting a mutation from inspiring English sources

They disrupted the function of DJ-1β by inserting a mutation into the middle of the gene.

Despite the efficiency of inserting a mutation that completely disrupts the function of a gene, most genetic factors in common complex diseases are expected to be noncrucial, coding single nucleotide polymorphisms or expression differences [ 55].

RAG−/− mice were produced by inserting a targeted mutation on chromosome 2, resulting in a "non-leaky" severe combined immune deficiency[12].

pcDNA3-HA-UCH L1 C90S and H161D mutants were generated by inserting a specific mutation at the Cys 90 and H161 sites in wild-type pcDNA3-HA-UCH L1 plasmid with the use of the Quik-Change Site Directed Mutagenesis Kit following the manufacturer's instructions (Stratagene).

The tat/rev shRNA target sequence is located on both gag-pol and on rev mRNAs so we reasoned that inserting a synonymous mutation in rev that would alter the shRNA target on the lentiviral helper plasmid, but would not change the Rev ORF, might increase titers.

To exclude, the changes that were possibly caused by inserting a splice mutation and/or 'humanization' of exon 47 (6), we additionally compared the expression profiles of the 6-week-old MPI11Q/11Q KI mice (n = 3) with their WT littermates (n = 3).

The final portion of the C. difficile CRISPR-Cas9 plasmid is the donor region which is necessary to insert a desired mutation.

Conventionally, homologous recombination is employed in gene targeting to insert a designed mutation into the homologous recombination loci in mouse ES cells.

The ATG16L1300A/300A-targeting construct inserted a point mutation in exon 9 resulting in the codon change ACT (threonine) to GCT (alanine).

Mutations inserting a single tryptophan residue as a spectroscopic probe at three different sites cause no perturbation in the protein's CD spectral characteristics, or in its quaternary structural status.

This is especially useful when handling the large fragments for recombination analyses, genomic mutation, or inserting a foreign open-reading frame into the infectious clone.