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All implants were inserted with the same recommended torque of <35 Ncm.
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Each is inserted with the hair cuticle going in the same direction, the way it's arranged naturally on heads, so that the hair doesn't tangle.
The positive control was the same vector inserted with the complementary sequence of miR-130a.
Inserts with the same CP frequencies as Min-E but without dinucleotide frequency constraints (Min-U; CpG: 0.82; UpA: 0.95) or where CpG and UpA frequencies were maximised (Min-H; CpG: 1.3; UpA: 0.98) were generated similarly.
As a control for positive charge, mutant strains with lysines inserted in the same positions as arginines were also generated.> -wrap-foot> testestheheffectsts of H2A NTD changes on chromatin compaction, the physical distance between two probes on chromosome XVI spaced 275 kb apart was assessed in each of the H2A mutants using fluorescent in situ hybridization (FISH).
In the improved manner, the valve plate was first encompassed with a thin layer of cotton soaked with MMC, then inserted into the same area.
In the traditional technique, 6 × 4 mm cotton soaked with MMC (0.25 0.33 mg/ml) was placed in the implantation area for 2 5mins; in the new technique, the valve plate first was encompassed with a thin layer of cotton soaked with MMC, then inserted into the same area.
We named the modified plasmids with the HS4 insulator sequence inserted in the same or opposite direction with the polh promoter as pFa-HS4 and pFa-HS4−, respectively.
In one patient implants were removed and new implants were inserted at the same site with continuing problems.
The product was digested with BamHI and inserted into the same site in pET28a.
The product was digested with BamHI and inserted into the same site in pET28a to generate pET28a/eIF4AIIIIP.
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