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A week later, they inserted viable embryos into surrogate mothers that carried the calves to term.
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Tests showed that the stem cells had migrated to the site of injury, even if inserted far away, and were viable.
h22 is a lethal EMS-induced null allele encoding a truncated protein of only 42 amino acids due to a premature stop codon; h674 is a lethal EMS-induced hypomorph allele encoding a protein lacking the C-terminal 103 amino acids due to a premature stop codon and h1J3 and h1 are viable alleles with inserted transposable elements ([26], [27]; Flybase).
To answer this question we randomly inserted a gene into a viable network and developed stable state vectors to test whether stability was retained.
For intravenous (IV) injection via tail vein, atrium FACS sorted cells that have been cultured for 5 passages were labeled with PKH26 (Sigma) which is a lipophilic marker inserted into the membrane of viable cells according to manufacturer's protocol prior to injection for better cell tracking [ 27].
All cells present in each insert were viable.
Surprisingly, mice in which Hras is inserted into the Kras locus are viable despite completely lacking Kras proteins [31].
External skin markers do not provide accurate intervertebral motion description [10, 11], and invasive positioning of markers inserted in the vertebrae is not generally viable.
EcRE-lacZ, a homozygous viable stock with seven EcREs inserted into a lacZ promoter was used to determine the pattern of ecdysone signalling (Koelle et al, 1991).
Therefore, we reserved the viable bone tissue as much as possible, and inserted an appropriately trimmed graft into the inter-vertebral disc space.
Mandibular arch distalization through TADs inserted in the retromolar area after extraction of the third molars appears to be a viable option when treating a dentoalveolar class III patient with lower anterior crowding.
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