The muon target is inserted upstream of the neutron target in the proton beam line.
Plasmid pACT contains a luciferase cassette driven by a minimal, enhancerless promoter, into which vector proviruses are inserted upstream for evaluation by luciferase assays and northern blots.
For the in vitro synthesis of gRNAs, the T7 promoter was inserted upstream of gRNAs by PCR using oligomers as previously described31, and 5′-GG transcription start region was arbitrarily created (Supplementary Table S4).
For C9RAN reporters, the 5′ end of C9rof72 intron1 was PCR-amplified from human fibroblast DNA and inserted upstream of previously published GGG-NL-3xF in pcDNA3.136 via NheI.
Upstream sequence (3.5 kb) including the first exon and intron up to the putative start codon were amplified via PCR and inserted upstream of the luciferase gene in a pGL3 reporter gene construct.
A 51-bp region of telomeric DNA inserted upstream of either HIS4 or ARG4 very strongly stimulates recombination.
Oligonucleotides corresponding to both the wild-type and mutated -3306 element were inserted upstream of a thymidine kinase luciferase construct.
The nebulizer system (Aerogen, Inc., Mountain View, CA, USA) was inserted upstream from the electrically heated humidifier [30 32].
To control essential gene expressions, tetracycline-regulated promoter (Ptet) was inserted upstream of the accBC genes, and gene expressions were regulated by the absence of doxycycline.
Briefly, the promoter region of the TDH3 gene, which allows constitutive expression at a high level, was fused with the URA3 marker gene and then inserted upstream of the start codon of the HAA1 gene.
An XbaI restriction site was inserted upstream of ss in a tail in the forward primer.
