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To confirm that the genomic gfp sequence had been converted into the yfp, PCR was conducted using primers (Table S1) specific for yfp and the 35S promoter driving the selectable marker gene (HPT), the latter expression unit being present within the stably inserted transfer DNA that also carries the gfp gene.
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F. Insert Transfer Tube pre-loaded with HDR fluid into EpiCollector.
It remains unclear whether or not specific entry vector/destination vector recombination reactions might affect the efficiency of insert transfer, but this will need to be tested by the end user on a case-by-case basis.
With TPS, a large majority of the cDNA clones constructed by using the Gateway-compatible cloning vector (pCMVFL3; Toyobo, Osaka, Japan) were sequenced by using a combination of TPS and insert transfer with Gateway technology to reduce the number of shotgun clones with transposons in the vector sequence [ 31].
Filled circles represent males that inserted and transferred sperm into the hermaphrodite.
All the workflows generated by W are then transformed to insert the transfer nodes whenever there is a inter-cloud edge in the workflow graph.
Additionally, each male was given up to 5 min to insert and transfer sperm.
Culture medium was aspirated and the inserts transferred to 12-well plates containing 1.5 ml/well donor buffer (DMEM without phenol red, 25 mM HEPES and 0.1% bovine serum albumin) and placed in an orbital shaker at 37 °C.
The DePhine spacecraft will be inserted into Mars transfer and will initially enter a Deimos quasi-satellite orbit to carry out a comprehensive global mapping.
Analysis showed a decrease in the LED maximum temperature of the finless lighting device from 99.8 °C to 82.1 °C when aluminum was inserted, enabling the transfer of heat energy accumulated inside the lighting structure, and lowering the overall temperature of the lighting system and extending its lifespan.
The target fragments of P1-2A and 3C were inserted into baculoviral transfer vector pVL1393 using BamH I/EcoR I and EcoR I/Bgl II sites following the routine protocols to generate a transfer plasmid.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com