28-gauge needle was inserted to following position: 1mm right of the midline, 0.2 mm posterior and 2.5 mm depth from bregma.
An unpaired gap is inserted to the position corresponding to the deleted column 4 in Ao.
A vaginal marker was carefully inserted to indicate the position of the vaginal apex, without distortion of the vagina.
Using this technique, the coaxial needle (17G, Bard Peripheral Vascular Inc., Tempe, AZ, USA) was usually inserted to the desired position during expiration.
To make the decoded sequence clearer, a period was inserted to indicate the origin position, where the two ends of the sequence were stitched together in the seeding process.
A trial femoral and tibial component of the prosthesis was inserted to check whether the positioning was adequate.
The β-sheet breaker N-α-Fmoc- N-α- 2-Fmoc-oxy-4-methoxybenzyl)- l-Phe (Fmoc-(FmocHmb)Phe-OH, N-α- 2-Fmoc-oxy-4-methoxybenzylsitioN-α- 2-Fmoc-oxy-4-methoxybenzylatioN-α- 2-Fmoc-oxy-4-methoxybenzylthe subsequeN-α- 2-Fmoc-oxy-4-methoxybenzylN-α- 2-Fmoc-oxy-4-methoxybenzyl-beN-α- 2-Fmoc-oxy-4-methoxybenzylmethylammonium hexafluorophosphate (HATU, Novabiochem) and DIPEA.
A mutant human class IV enzyme was engineered in which a Gly residue was inserted at position 117 (G117ins).
The LAGLIDADG sequences of this clade originated from introns inserted at position 888.
In this mutant, the transposon was inserted at position 1,625 of pld1 gene.
In addition, two adjacent HA tags were inserted at positions 243 (5b), 403 (8b), and 504 (11b) to increase the immunofluorescence signal.
