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With bibloc devices, the lateral attachments producing mandibular propulsion can be inserted to generate traction or compression.
By adopting a traditional generation method for the coastline echo under small radar range, the time for the scan line to rotate around is much more likely to exceed three seconds, since extra sampling coordinate points (if available) have to be inserted to generate a high quality coastline echo.
Into the MCS-B site, separated from the MCS-A by an IRES sequence, the DHFR gene was inserted to generate the pIRES-DHFR vector (Fig. 1A).
To provide chemical specificity, protoporphyrin IX (PPIX), the penultimate molecule in the heme synthetic pathway into which a ferrous iron is inserted to generate heme, was used to increase heme synthesis[34].
Then, GFP and SV40 terminator sequences were inserted to generate pBacGFP-3xP3DsRed.
The GFP cassette of FUGW was removed and mCherry cDNA inserted to generate FUmChW.
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Briefly, cells were plated 24 h after transfection onto 24-well plates that contained inserts to generate defined 'scratch areas'.
Homologous recombination of the 82 kb overlapping region and the common sequence of the BGM vectors resulted in the extension of the insert to generate Tg-250SB.
The DNA inserts to obtain fusions downstream the Venus fragment were cloned into XhoI-XbaI sites, while inserts to generate fusions upstream the Venus fragment were cloned into EcoRI-XhoI sites.
To make a genome-wide assessment of gene essentiality, we used random in vitro transposition using two transposon inserts to generate two transposon libraries in C. jejuni 11168 (see Materials and Methods).
The normalized cDNA library was constructed according to method 4 of [72], except that Qiagen Taq DNA polymerase (Qiagen, Inc., CA, USA) was the polymerase used for PCR amplification of cDNA inserts to generate the driver population.
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