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Three months later following another shunt obstruction, the shunt was removed and a Rickham's reservoir inserted to gauge shunt dependence.
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A flexible balloon-tipped rectal catheter (20 French gauge) was inserted to insufflate approximately 3 litres of CO2 gas into the colon, using an automated insufflator (ProtoCO2l, Bracco Diagnostics, Princeton, New York, USA).
28-gauge needle was inserted to following position: 1mm right of the midline, 0.2 mm posterior and 2.5 mm depth from bregma.
A 28 gauge dummy cannula was inserted to prevent clogging of the guide cannula.
The patients were not premedicated, and a 20-gauge venous cannula was inserted to administer Ringer's lactated solution.
Using only landmarks to guide needle insertion, the physician inserted a 22 gauge 3.5 inch spinal needle into the suspected bursa.
As shown in Fig. 4, in vivo PA imaging simultaneously detected both the methylene blue uptake in the SLN and the inserted 18 gauge needle.
The trachea was exposed, a tracheotomy was performed to insert a 18-gauge needle connected to a computer-controlled small-animal ventilator.
The plastic sheath with stylet was then inserted adjacent to the 19-gauge needle and into the small bowel and was subsequently snared to facilitate guidewire passage.
Once the gauge is inserted in to the gap, tighten the adjusting screw gradually while sliding the gauge back and forth until there is a noticeable "drag".
After allowing time for the anesthetic to take effect, an attempt was made to insert a 17-gauge Touhy needle into the skin.
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