This plasmid contained a T7 RNA polymerase promoter upstream of the insert to drive transcription by this enzyme.
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The genes on the array included whole chicken genome, 150 chicken microRNAs, [ 72, 134, 135], all known MDV and two avian influenza virus (H5N2 and H5N3) transcripts [ 72]. 500 ng of total RNA was reverse transcribed into cDNA with a T7 sequence inserted in cDNA to drive the synthesis of complementary RNA (cRNA).
Furthermore, once a helix has inserted, this will help to drive the curvature preferred by the next Arf molecules.
Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the xlFlk1 promoter.
The 31 Promoterome inserts that failed to drive GFP expression may be from inactive genes, may contain promoters with activity that is male-specific, only induced under specific environmental conditions, or too weak to be observed by the method followed, or the gene model used in selecting the promoter region may be wrong.
The PCR products were digested with BamHI and ligated into the transformation vector pU1301, which contained a maize ubiquitin gene promoter to drive the inserted gene [ 12].
The APOA-II gene is expressed in liver and intestine, and we previously constructed a lentivector in which we inserted APOA-II regulatory sequences to drive GFP expression, and confirmed its functionality both in vitro and in vivo[ 12, 13].
This plasmid retains a strong gpdA promoter to drive the insert's gene expression and Bar resistance coding sequence to express the selectable marker that would provide resistance to phosphinothricin (PPT).
After drilling the defect to the required depth, an extractor was inserted and driven down to the bottom of the defect.
